|本期目录/Table of Contents|

[1]钱江,陈锦珊,文磊,等.麻风树叶提取物体外抗蠕形螨活性及皮肤安全性的实验研究[J].传染病信息,2017,06:355-357.
 QIAN Jiang,CHEN Jin-shan,WEN Lei,et al.Anti-demodecidosis activity and skin safety of Jatropha curcas.l leaves extract in vitro[J].Infectious Disease Information,2017,06:355-357.
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麻风树叶提取物体外抗蠕形螨活性及皮肤安全性的实验研究(PDF)

《传染病信息》[ISSN:1007-8134/CN:11-3886/R]

期数:
2017年06期
页码:
355-357
栏目:
论著
出版日期:
2017-12-20

文章信息/Info

Title:
Anti-demodecidosis activity and skin safety of Jatropha curcas.l leaves extract in vitro
作者:
钱江陈锦珊文磊
363000 漳州,解放军第一七五医院 厦门大学附属东南医院皮肤科(钱江、陈锦珊、洪小兰、田雅兰);361000,厦门大学医学院中医系(文磊)
Author(s):
QIAN Jiang CHEN Jin-shan WEN Lei HONG Xiao-lan TIAN Ya-lan
Department of Dermatology, 175 Hospital of PLA/ Affiliated Southeast Hospital of Xiamen University, Zhangzhou 363000, China
关键词:
麻风树叶蠕形螨抗螨
Keywords:
Jatropha curcas.l leaves demodecidosis anti-demodecidosis
分类号:
R757.3
DOI:
10.3969/j.issn.1007-8134.2017.06.010
文献标识码:
A
摘要:
目的 探讨麻风树叶治疗蠕形螨病的应用价值。方法 用80% 乙醇热回流提取法提取麻风树叶提取液。取蠕形螨感染者面部皮脂,分离并鉴定蠕形螨备用。设不同浓度麻风树叶实验组、2% 浓度的甲硝唑对照组和生理盐水对照组,进行体外抗螨实验。pH 仪测定不同浓度麻风树叶提取物pH 值。设麻风树叶实验组和75% 乙醇对照组,用健康家兔进行皮肤刺激实验和急性皮肤毒性实验。结果 50、25、12 mg/ml 麻风树叶组与2% 甲硝唑组毛囊蠕形螨死亡时间分别为(1.55±0.67)min、(1.61±0.67)min、(2.47±0.80)min 和(1.20±0.48) min。50、25 mg/ml 麻风树叶组以及2% 甲硝唑组两两之间差异无统计学意义(P 均> 0.05)。50、25、12 mg/ml 麻风树叶提取液pH 值分别为6.07±0.73、6.27±0.82、6.35±0.83,对家兔完整皮肤及破损皮肤刺激评分均为0,且无明显毒性。结论 麻风树叶提取物具有较强的体外抗蠕形螨活性且具有皮肤安全性。
Abstract:
Objective To test the application value of the extract of Jatropha curcas.l leaves in treatment of demodecidosis. Methods Jatropha curcas.l leaves were extracted with 80% ethanol by using heat reflux method. Facial sebum specimen fromdemodecidosis-infected patients were used to isolate and identify demodecidosis. Different concentration groups of Jatropha curcas.l leaves,2% metronidazole control group and physiological saline control group were defined. The anti-demodecidosis experiment was performed in vitro. The pH value of different concentrations of Jatropha curcas.l leaves extract was determined. Skin irritation test and acute skintoxicity test were carried out in healthy rabbits, and Jatropha curcas.l leaves and 75% ethanol served as experiment and control groups,respectively. Results The mite-killing time was (1.55±0.67) min with 50 mg/ml Jatropha curcas.l leaves extract, (1.61±0.67) minwith 25 mg/ml Jatropha curcas.l leaves extract, (2.47±0.80) min with 12 mg/ml Jatropha curcas.l leaves extract, and (1.20±0.48)min with 2% metronidazole. There was no significant different between 50, 25 mg/ml Jatropha curcas.l leaves extract groups and 2%metronidazole group (P > 0.05). The pH value was 6.07±0.73 of 50 mg/ml Jatropha curcas.l leaves extract, 6.27±0.82 of 25 mg/ml Jatrophacurcas.l leaves extract and 6.35±0.83 of 12 mg/ml Jatropha curcas.l leaves extract. Score for irritation to normal and wounded rabbit skinwas both 0, and acute toxicity test showed no significant toxicity. Conclusions Jatropha curcas.l leaves extract shows a remarkableactivity to demodecidosis with skin safety in vitro.

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备注/Memo

备注/Memo:
[基金项目] 漳州市自然科学基金(ZZ2014J33)
更新日期/Last Update: 2017-12-20