|本期目录/Table of Contents|

[1]李琳,钟青.溃疡性结肠炎患者肠道菌群变化与细胞因子、TLRs 分子表达的相关性研究[J].传染病信息,2017,06:361-364.
 Li Lin,ZHONG Qing*.Correlation of intestinal microflora with cytokines andToll-like receptors expression in patients with ulcerative colitis[J].Infectious Disease Information,2017,06:361-364.
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溃疡性结肠炎患者肠道菌群变化与细胞因子、TLRs 分子表达的相关性研究(PDF)

《传染病信息》[ISSN:1007-8134/CN:11-3886/R]

期数:
2017年06期
页码:
361-364
栏目:
论著
出版日期:
2017-12-20

文章信息/Info

Title:
Correlation of intestinal microflora with cytokines andToll-like receptors expression in patients with ulcerative colitis
作者:
李琳钟青
272033 济宁,山东省医学科学院 山东省寄生虫病防治研究所消化内科(李琳) ;650041,昆明市第三人民医院肝病二科(钟青)
Author(s):
Li Lin ZHONG Qing*
Digestive System Department, Shandong Institule of Parasitical Disease, Shandong Academy of Medical Science, Jining 272033, China
关键词:
溃疡性结肠炎肠道菌群炎性细胞因子炎症反应
Keywords:
ulcerative colitis intestinal flora inflammatory cytokines inflammatory response
分类号:
R574.62;R378
DOI:
10.3969/j.issn.1007-8134.2017.06.012
文献标识码:
A
摘要:
目的 探讨溃疡性结肠炎患者肠道菌群变化与细胞因子、TOLL 样受体(Toll-like receptors, TLRs)分子表达的相关性。方法 将2015 年6 月—2016 年12 月在山东省医学科学院第三附属医院确诊并接受治疗的溃疡性结肠炎患者78例作为试验组,同时选择未患溃疡性结肠炎的80 例健康者作为对照组。分别对试验组和对照组进行肠道菌群检测,肠黏膜TLR2、TLR4、TLR5、TLR9 分子表达检测和外周血IL-4、IL-6、IL-17、IL-23、TNF-α 等炎性细胞因子表达检测,分析炎性细胞因子和TLRs 表达与肠道菌群变化的关系。结果 试验组双歧杆菌、乳杆菌含量明显低于对照组(P 均< 0.05),拟杆菌、肠杆菌、肠球菌、梭杆菌含量明显高于对照组(P 均< 0.05);试验组肠黏膜组织中TLR2、TLR4、TLR5、TLR9 表达明显高于对照组(P 均< 0.05);试验组外周血IL-4 表达低于对照组,IL-6、IL-17、IL-23、TNF-α 等炎性细胞因子表达高于对照组(P 均< 0.05)。Pearson 相关性分析显示,TLR2、TLR4、TLR5、TLR9 表达与拟杆菌、肠杆菌、肠球菌含量呈正相关,与双歧杆菌、乳杆菌含量呈负相关;与IL-6、IL-17、IL-23、TNF-α 表达呈正相关,与IL-4 表达呈负相关。结论 溃疡性结肠炎患者正常肠道菌群平衡被打破,促炎因子表达增加,抑炎因子表达减少,TLRs 分子表达增加。肠道菌群紊乱可能通过增强TLRs 分子表达来促进促炎因子的分泌,介导肠黏膜炎性反应。
Abstract:
Objective To investigate the correlation of the changes of intestinal flora with the expression of cytokines and Tolllikereceptors (TLRs) molecules in patients with ulcerative colitis. Methods From June 2015 to December 2016, 78 patients withulcerative colitis diagnosed and treated in the Third Affiliated Hospital of Shandong Academy of Medical Sciences were selected as theexperimental group, and 80 healthy subjects were chosen as the control group. The intestinal flora detection, expression of TLR2, TLR4,TLR5, TLR9 molecules in intestinal mucosa, expression of IL-4, IL-6, IL-17, IL-23, TNF-α and other inflammatory cytokines inperipheral blood in the experimental group and control group were analyzed respectively. The correlation of inflammatory cytokines andthe expression of TLRs with the change of intestinal flora was also analyzed. Results The contents of bifidobacterium and lactobacillusin the experimental group were significantly lower than those in the control group (P < 0.05), while the contents of bacteroides,enterobacter, enterococcus and fusobacterium were significantly higher (P < 0.05). The expressions of TLR2, TLR4, TLR5 and TLR9in intestinal mucosa of the experimental group were significantly higher than those of the control group (P < 0.05). The expression ofIL-4 in peripheral blood of the experimental group was lower than that of the control group, and the expressions of IL-6, IL-17, IL-23,TNF-α and other inflammatory cytokines were higher than that of the control group (P < 0.05). Pearson correlation analysis showed thatTLR2, TLR4, TLR5, TLR9 expressions were positively correlated with the contents of bacteroides, enterobacter and enterococcus, andnegatively correlated with bifidobacterium and lactobacillus; it was also positively correlated with IL-6, IL-17, IL-23, TNF-α expressionand negatively correlated with IL-4 expression. Conclusions Normal intestinal flora balance of ulcerative colitis patients is broken,the expression of proinflammatory cytokines increases, the expression of proinflammatory factors decreases, and the expression of TLRsmolecules increases. The disorder of intestinal flora promotes the secretion of proinflammatory cytokines by enhancing the expression ofTLRs molecules and mediates the inflammatory response of the intestinal mucosa.

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备注/Memo

备注/Memo:
[通信作者] 钟青,E-mail: o1r1tp@163.com
更新日期/Last Update: 2017-12-20