|本期目录/Table of Contents|

[1]唐子淋,刘妍,刘佳梁,等.HBsAg/HBsAb 双阳性慢性乙型肝炎患者HBV S 基因免疫逃逸相关变异分析[J].传染病信息,2018,02:116-120.
 TANG Zi-lin,LIU Yan*,LIU Jia-liang,et al.Analysis of HBV S gene immune escape-related mutation in chronic hepatitis B patients with HBsAg/HBsAb co-existence[J].Infectious Disease Information,2018,02:116-120.
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HBsAg/HBsAb 双阳性慢性乙型肝炎患者HBV S 基因免疫逃逸相关变异分析(PDF)

《传染病信息》[ISSN:1007-8134/CN:11-3886/R]

期数:
2018年02期
页码:
116-120
栏目:
专题论著
出版日期:
2018-03-20

文章信息/Info

Title:
Analysis of HBV S gene immune escape-related mutation in chronic hepatitis B patients with HBsAg/HBsAb co-existence
作者:
唐子淋刘妍刘佳梁思兰兰李乐廖昊邵金曼徐东平李进
541004, 桂林医学院临床学院( 唐子淋); 100039 北京,解放军第三〇二医院临床研究管理中心/ 全军传 染病研究所(刘妍、刘佳梁、思兰兰、李乐、廖昊、邵金曼、 徐东平),医务部(李进)
Author(s):
TANG Zi-lin LIU Yan* LIU Jia-liang SI Lan-lan LI Le LIAO Hao SHAO Jin-man Xu Dong-ping LI Jin*
Clinical Medical School, Guilin Medical University, 541004, China
关键词:
慢性乙型肝炎乙型肝炎表面抗原乙型肝炎表面抗体S 基因免疫逃逸相关变异
Keywords:
chronic hepatitis B HBsAg HBsAb S gene immune escape-related mutation
分类号:
R512.62
DOI:
10.3969/j.issn.1007-8134.2018.02.006
文献标识码:
A
摘要:
目的 分析HBsAg/HBsAb 双阳性慢性乙型肝炎患者HBV S 基因主要亲水区免疫逃逸相关位点变异特点。 方法 收集89 例HBsAg/HBsAb 双阳性和148 例HBsAg 单阳性的慢性乙型肝炎患者血清及临床资料,提取患者血清HBV DNA,扩增患者HBV S 基因并进行测序,应用DNASTAR Lasergene MegAlign 软件对主要亲水区已有文献报道的46 个免疫 逃逸相关位点及新增N- 糖基化变异进行比对分析。结果 2 组患者在年龄、ALT、TBIL、HBV DNA 载量和HBeAg 阳性率 方面差异均无统计学意义(P 均< 0.05)。HBsAg/HBsAb 双阳性患者HBV S 基因主要亲水区变异的总检出率为31.46%, 明显高于HBsAg 单阳性患者的18.92%(P < 0.05),其中sL110I/S、sT113N/S、sT131I/N/P 和sS143L/M/T 的变异检出率 明显高于单阳性组;双阳性患者多位点联合变异检出率亦明显高于单阳性患者(20.22% vs. 6.08%,P < 0.05)。双阳性 患者新增N- 糖基化变异检出率高于单阳性患者(7.87% vs. 2.03%),差异具有统计学意义(P < 0.05)。结论 HBsAg/ HBsAb 双阳性患者比HBsAg 单阳性患者的HBV S 基因免疫逃逸相关变异种类更多,单位点和多位点联合变异检出率 更高,并且新增N- 糖基化变异检出率也更高,这些变异可能是引起慢性乙型肝炎患者HBsAg/HBsAb 双阳性共存的驱动 因素之一。 
Abstract:
Objective To analyze the mutation characteristics of the immune escape-related sites of HBV S gene major hydrophilic regions in chronic hepatitis B patients with HBsAg and HBsAb co-existence. Methods The serum samples and clinical data of 89 patients with HBsAg/HBsAb co-existence and 148 single HBsAg positive patients with chronic hepatitis B were collected. Serum HBV DNA was extracted from the included patients, and HBV S genes were amplified from serum and then sequenced. Forty-six well-documented immune escape-related site mutation and additional N-glycosylation mutation alignments in major hydrophilic regions were analyzed using DNASTAR Lasergene MegAlign software. Results There were no statistically significant differences in age, ALT, TBIL, HBV DNA level and HBeAg positive rate between the 2 groups. The overall detection rate of immune escape-related mutations of HBV S gene major hydrophilic regions in co-existence group was 31.46%, which was significantly higher than that in HBsAg single positive group (18.92%, P < 0.05). The detection rates of sL110I/S mutation, sT113N/S mutation, sT131I /N/P mutation and sS143L/M/ T mutation were significantly higher than those in the single positive group, and the detection rates of multiple mutations in co-existence group were also significantly higher than those in the single positive group (20.22% vs. 6.08%, P < 0.05). In addition, the detection rate of additional N-glycosylation mutations in co-existence group was higher than that in single positive group (7.87% vs. 2.03%), the difference was statistically significant (P < 0.05). Conclusions HBV S gene in HBsAg/HBsAb co-existence patients harbours more mutational diversity, higher detection rate of single and multiple mutation, and higher detection rate of additional N-glycosylation mutations than those in HBsAg single positive patients. These mutations may be one of the driven factors of HBsAg and HBsAb co-existence in chronic hepatitis B patients.  

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备注/Memo

备注/Memo:
[基金项目] 北京市自然科学基金(7172206);国家自然科 学基金(81572010,81373136);首都卫生发展科研专项项目 (2016-2-5032)
[通信作者] 刘妍,E-mail: liuyan5360@163.com;李进,E-mail: lijin302@hotmail.com
更新日期/Last Update: 2018-03-20