|本期目录/Table of Contents|

[1]杨帆,杨涛,周文靖,等.巨噬细胞外泌体抑制HBV DNA 复制[J].传染病信息,2018,02:125-130.
 YANG Fan,YANG Tao,ZHOU Wen-jing,et al.Macrophage-derived exosomes inhibit HBV DNA replication[J].Infectious Disease Information,2018,02:125-130.
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巨噬细胞外泌体抑制HBV DNA 复制(PDF)

《传染病信息》[ISSN:1007-8134/CN:11-3886/R]

期数:
2018年02期
页码:
125-130
栏目:
专题论著
出版日期:
2018-03-20

文章信息/Info

Title:
Macrophage-derived exosomes inhibit HBV DNA replication
作者:
杨帆杨涛周文靖徐若男王福生
233000,蚌埠医学院基础免疫系(杨帆、周文靖、 王福生);100039 北京,解放军第三〇二医院感染病诊疗与 研究中心(杨涛、徐若男、王福生)
Author(s):
YANG Fan YANG Tao ZHOU Wen-jing XU Ruo-nan WANG Fu-sheng*
Department of Basic Immunology, Bengbu Medical College, 233000, China
关键词:
外泌体miRNA-638巨噬细胞慢性乙型肝炎
Keywords:
exosomes miRNA-638 macrophages chronic hepatitis B
分类号:
R512.62
DOI:
10.3969/j.issn.1007-8134.2018.02.008
文献标识码:
A
摘要:
目的 探讨巨噬细胞(macrophage, M?)外泌体抑制HBV DNA 复制的机制及与慢性乙型肝炎(chronic viral hepatitis B, CHB)肝损伤的相关性。方法 体外分离鉴定M? 外泌体,并将其与HepG2.2.15 细胞共培养,利用实时 定量 PCR 检测共培养后HBV DNA 复制的变化,分别分析M? 及其上清外泌体中微小RNA(microRNA, miRNA)-638 的表达;同时入组CHB 免疫活化(immune activation, IA)期、低(非)复制(inactive carrier, IC)期患者 ,并以同 期健康者作为对照组,通过实时定量PCR 检测血清外泌体中miRNA-638 的表达,并与肝损伤、HBV DNA 载量进行相 关性分析。 结果 M? 外泌体可以抑制HBV DNA 复制;M? 及其上清外泌体中miRNA-638 高表达;CHB 患者IA 期 miRNA-638 的表达水平低于IC 期;CHB 患者血清外泌体中miRNA-638 的表达水平与ALT、AST 和HBV DNA 水平呈负相关。 结论 M? 外泌体可以抑制HBV DNA 复制,外泌体中miRNA-638 的表达水平与肝脏炎性损伤和病毒复制密切相关,M? 外泌体相关miRNA 可能是潜在的抑制HBV 复制、减轻肝脏炎症的靶点。
Abstract:
Objective To investigate the mechanism of macrophage-derived exosomes on inhibiting HBV replication and analyze its relationship with liver damage in chronic hepatitis B (CHB) patients. Methods Macrophage-derived exosomes were isolated in vitro and co-cultured with HepG2.2.15 cells. Real-time quantitive PCR was used to detect the change of HBV DNA replication after coculture, and the expression levels of microRNA-638 (miRNA-638) in the exosomes isolated from macrophages and supernatant were analyzed. In addition, CHB patients at immune activation (IA) and inactive carrier (IC) stage, as well as healthy controls were included in this study. The miRNA-638 expression level in macrophage-derived exosomes was detected with real-time quantitive PCR, and its correlation with liver damage and HBV DNA load was analyzed. Results Exosomes derived from macrophages could inhibit HBV DNA replication, and miRNA-638 expression upregulated in the exosomes isolated from macrophages and supernatant; miRNA-638 expression decreased in the CHB patients at IA stage compared with that at IC stage; miRNA-638 expression was negatively correlated with ALT, AST, and HBV DNA in the serum exosomes of CHB patients. Conclusions Exosomes derived from macrophage may inhibit HBV DNA replication. miRNA-638 expression in exosomes is closely associated with liver inflammation injury and viral replication. Exosomes associated miRNA in macrophages may be a potential target for inhibiting HBV replication and reducing liver inflammation.  

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备注/Memo

备注/Memo:
[基金项目] 国家自然科学基金青年项目(81400626)
[通信作者] 王福生,E-mail: fswang302@163.com
更新日期/Last Update: 2018-03-20