|本期目录/Table of Contents|

[1]张敬霞,曲芬.飞行时间质谱技术鉴定丝状真菌的研究进展[J].传染病信息,2018,02:171-175.
 ZHANG Jing-xia,QU Fen*.Research progress of identification of filamentous fungi by MALDI-TOF MS[J].Infectious Disease Information,2018,02:171-175.
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飞行时间质谱技术鉴定丝状真菌的研究进展(PDF)

《传染病信息》[ISSN:1007-8134/CN:11-3886/R]

期数:
2018年02期
页码:
171-175
栏目:
综述
出版日期:
2018-03-20

文章信息/Info

Title:
Research progress of identification of filamentous fungi by MALDI-TOF MS
作者:
张敬霞曲芬
075000 张家口,河北北方学院检验学院(张敬霞) ; 100039 北京,解放军第三〇二医院临床检验中心(曲芬)
Author(s):
ZHANG Jing-xia QU Fen*
Inspection College of HeBei Beifang University, Zhangjiakou 075000, China
关键词:
丝状真菌基质辅助激光解析电离飞行时间质谱影响因素鉴定效果
Keywords:
filamentous fungi matrix-assisted laser-desorption/ionization time of flight mass spectrometry influencing factors identification effect
分类号:
R446.5
DOI:
10.3969/j.issn.1007-8134.2018.02.019
文献标识码:
A
摘要:
侵袭性真菌感染与免疫力低下患者的高发病率和高病死率密切相关,其早期诊治对提高患者救治率至关重要。 基质辅助激光解析电离飞行时间质谱(matrix-assisted laser-desorption/ionization time of flight mass spectrometry, MALDI-TOF MS)技术通过检测真菌中固有的特异性蛋白从而实现对丝状真菌快速、准确的鉴定。本文从技术原理、标本前处理、鉴定 效果及影响因素等方面对MALDI-TOF MS 技术鉴定丝状真菌的研究进展作一综述。
Abstract:
Invasive fungal infections are associated with higher morbidity and higher mortality in patients with hypoimmunity. The early diagnosis and treatment is essential for improving the treatment rate. Matrix-assisted laser-desorption/ionization time of flight mass spectrometry (MALDI-TOF MS) technology realizes a rapid and accurate identification of fungi by detecting the fingerprint of specific ribosomal subunit proteins inherent in fungus. This paper reviews the research progress of MALDI-TOF MS identification of filamentous fungi in terms of technical principle, pretreatment of samples, identification effect and influencing factors.

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备注/Memo

备注/Memo:
[基金项目] 北京市科学技术委员会首都市民健康培育项目 (Z151100003915151)
[通信作者] 曲芬,E-mail: QF302@163.com
更新日期/Last Update: 2018-03-20