|本期目录/Table of Contents|

[1]冯彦军,李小红,凌 寅,等.痰结核分枝杆菌DNA 检测诊断肺结核的临床意义[J].传染病信息,2018,04:342-344.
 FENG Yan-jun,LI Xiao-hong,LING Yin,et al.Clinical significance of sputum Mycobacterium tuberculosis DNA in the diagnosis of pulmonary tuberculosis[J].Infectious Disease Information,2018,04:342-344.
点击复制

痰结核分枝杆菌DNA 检测诊断肺结核的临床意义(PDF)

《传染病信息》[ISSN:1007-8134/CN:11-3886/R]

期数:
2018年04期
页码:
342-344
栏目:
论 著
出版日期:
2018-08-30

文章信息/Info

Title:
Clinical significance of sputum Mycobacterium tuberculosis DNA in the diagnosis of pulmonary tuberculosis
作者:
冯彦军李小红凌 寅王霞芳叶志坚吴妹英
215000, 苏州大学附属传染病医院结核病科 (冯彦军、凌寅、王霞芳、叶志坚、吴妹英),传染病科(李小红)
Author(s):
FENG Yan-jun LI Xiao-hong LING Yin WANG Xia-fang* YE Zhi-jian WU Mei-ying
Tuberculosis Department, Fifth People's Hospital of Suzhou City, 215000, China
关键词:
结核分枝杆菌DNA 检测肺结核诊断
Keywords:
Mycobacterium tuberculosis DNA test pulmonary tuberculosis diagnosis
分类号:
R52
DOI:
10.3969/j.issn.1007-8134.2018.04.010
文献标识码:
A
摘要:
目的 评估痰结核分枝杆菌DNA 检测诊断肺结核的可行性及临床意义。方法 回顾性分析2016 年5 月— 12 月在我科住院的经痰结核分枝杆菌培养确诊的136 例肺结核患者的临床资料,比较痰涂片找抗酸杆菌和痰结核分枝杆 菌DNA 检测2 种方法的检出率。结果 136 例确诊的肺结核患者中,痰涂片阳性的有64 例(47.06%),痰结核分枝杆 菌DNA 检测阳性的88 例(64.71%),痰结核分枝杆菌DNA 检测阳性率明显高于痰涂片找抗酸杆菌(P < 0.05)。72 例 痰涂片阴性患者中痰结核分枝杆菌DNA 检测阳性29 例,占痰涂片阴性肺结核患者比例的40.28%。结论 痰结核分枝杆 菌DNA 检测优于痰涂片找抗酸杆菌,能够辅助涂阳肺结核的诊断,并提高涂阴肺结核的诊断水平,是一种快速、敏感的 病原学诊断方法。  
Abstract:
Objective To evaluate the feasibility and clinical significance of sputum Mycobacterium tuberculosis DNA in the diagnosis of pulmonary tuberculosis. Methods We retrospectively analyzed 136 patients with tuberculosis who were hospitalized in our department from May 2016 to December 2016, to compare the detection rate of sputum smear looking for Acidfast bacillus and sputum Mycobacterium tuberculosis DNA test methods. Results Sixty-four cases (47.06%) were positive for sputum smear and 88 cases (64.71%) were positive for sputum Mycobacterium tuberculosis among 136 patients with confirmed pulmonary tuberculosis. The positive detection rate of Mycobacterium tuberculosis DNA was significantly higher than that of sputum smear (P < 0.05). Among the 72 sputum smearnegative patients, 29 cases were positive for sputum Mycobacterium tuberculosis DNA, accounting for 40.28% of the total number of sputum smear-negative pulmonary tuberculosis patients. Conclusions Mycobacterium tuberculosis DNA detection is superior to sputum smear looking for Acidfast bacillus. It can assist the diagnosis of smear-positive pulmonary tuberculosis and increase the diagnosis rate of smear-negative pulmonary tuberculosis. It is a rapid and sensitive etiological diagnosis method.

参考文献/References


[1] 中华人民共和国国家卫生和计划生育委员会. 肺结核诊断[J]. 传染病信息,2017,30 (6):I-XII.
[2] World Health Organization. Global tuberculosis report 2016[M]. Geneva: WHO press, 2016.
[3] 卫生部疾病预防控制局,卫生部医政司,中国疾病预防控制 中心. 中国结核病防治规划实施工作指南(2008 年版)[M]. 北京:中国协和医科大学出版社,2008:1.
[4] 阳仕雄,阳咏芳,付汉东,等. 结核病诊断技术方法的选 择及实验室诊断路径[J].国际检验医学杂志,2017, 38(19):2728-2730.
[5] 杨永辉,朱桂云,陈宁. 结核分枝杆菌分子病理学诊断技术 进展[J].临床荟萃,2016,31(10):1063-1066.
[6] 邬小薇. 3 种方法检测结核分枝杆菌的比较[J]. 重庆医学, 2005,34(10):1506-1511.
[7] 廖传玉,蒋克珉,高万. 痰分枝杆菌快速培养和药敏试验的 培养[J]. 临床肺科杂志,2005,10(1):115.
[8] Qiftci IH, Karakece E. Comparative evaluation of TK SLC-L, a rapid liquid mycobacterial culture medium,with the MGIT system [J]. BMC Infect Dis, 2014, 14:130.
[9] 张洁,邢青,易俊莉,等. 传统培养法与PCR- 荧光探针 法鉴定分枝杆菌的对比研究[J]. 中国防痨杂志,2015, 37(3):291-294.
[10] 李幸彬,张广宇,杜秀然,等.防污染支气管肺泡灌洗对 痰菌阴性肺结核的诊断价值[J]. 中国防痨杂志,2008, 30(5):421-423.
[11] World Health Organization. Automated real-time nucleic acid amplification technology for rapid and simultaneous detection of tuberculosis and rifampicin resistance. Xpert MTB/RIF assay for the diagnosis of pulmonary and ertrapulmonary TB in adults and children: policy update[M]. Geneva: World Health Organization, 2013:10-17.
[12] 梁建琴,高华方,李洪敏,等. PCR- 荧光探针法检测临床 痰标本结核分枝杆菌的可靠性研究[J]. 中国防痨杂志, 2012,34(5):271-274.

备注/Memo

备注/Memo:
[ 基金项目] 苏州市科技局民生科技项目(SS201656);苏 州市临床重点病种诊疗技术专项(LCZX201514);苏州市重 点学科(SZZK201514) [ 作者单位] 215000, 苏州大学附属传染病医院结核病科 (冯彦军、凌寅、王霞芳、叶志坚、吴妹英),传染病科(李小红) [ 通信作者] 王霞芳,E-mail: 281805128@qq.com
更新日期/Last Update: 2018-09-08