|本期目录/Table of Contents|

[1]韩 冰,吴翠萍,赵 芯,等.数字PCR 和荧光定量PCR 诊断 急性期布鲁菌病的灵敏度比较初步研究[J].传染病信息,2019,04:312-316.
 HAN Bing,WU Cui-ping,ZHAO Xin,et al.A preliminary study on the sensitivity of digital PCR and fluorescence quantitative PCR in the diagnosis of acute brucellosis[J].Infectious Disease Information,2019,04:312-316.
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数字PCR 和荧光定量PCR 诊断 急性期布鲁菌病的灵敏度比较初步研究(PDF)

《传染病信息》[ISSN:1007-8134/CN:11-3886/R]

期数:
2019年04期
页码:
312-316
栏目:
论著
出版日期:
2019-09-12

文章信息/Info

Title:
A preliminary study on the sensitivity of digital PCR and fluorescence quantitative PCR in the diagnosis of acute brucellosis
文章编号:
 1007-8134(2019)04-0312-05
作者:
韩 冰吴翠萍赵 芯刘贵明蒋荣猛
100015,首都医科大学附属北京地坛医院感染二科(韩冰、蒋荣猛);100097,北京农业生物技术研究中心基因组测序及大数据挖掘课题组(赵芯、刘贵明);262500,潍坊益都中心医院感染科(吴翠萍)前两位作者对本文有同等贡献,均为第一作者
Author(s):
HAN Bing WU Cui-ping ZHAO Xin LIU Gui-ming JIANG Rong-meng*
Department 2 of Infection, Beijing Ditan Hospital Affiliated to Capital Medical University, 100015, China Department of Infection, Weifang Yidu Central Hospital, 262500, China HAN Bing and WU Cui-ping are the first authors who contributed equally to the article
* Corresponding author, E-mail: 13911900791@163.com
关键词:
布鲁菌数字PCR荧光定量PCR诊断
Keywords:
    Brucella digital PCR fluorescent quantitative PCR diagnosis
分类号:
 R516.7
DOI:
10.3969/j.issn.1007-8134.2019.04.006
文献标识码:
A
摘要:
 目的 建立用于布鲁菌检测的数字PCR 技术,初步用小样本开展研究,比较数字PCR 和荧光定量PCR 的灵 敏度差异,验证数字PCR 作为急性期布鲁菌病诊断技术的可行性。方法 选取符合我国急性期布鲁菌病诊断标准的20 例患 者的血清标本,分别用数字PCR 和荧光定量PCR 技术进行检测,初步比较2 者在急性期布鲁菌病诊断中的灵敏度差异,并 分析差异原因。结果 20 例急性期布鲁菌病患者血清样本中,数字PCR 检测阳性20 例,荧光定量PCR 检测阳性0 例。 结论 初步证实在急性期布鲁菌病患者血清标本检测中,数字PCR 检测灵敏度高于荧光定量PCR,有良好的临床应用前景, 但也存在一定局限性,尚须进一步扩大样本完成实验诊断技术临床研究,并进行多实验室验证。 
Abstract:
Objective A digital PCR technology for detection of Brucella was established. A preliminary study was carried out with small samples. To compared the sensitivity differences between digital PCR and fluorescence quantitative PCR and verify the feasibility of digital PCR as a diagnostic technique for acute brucellosis. Methods Serum samples from 20 patients who met the diagnostic criteria of acute brucellosis in China were detected by digital PCR and fluorescent quantitative PCR respectively. The sensitivity differences between the 2 methods in the diagnosis of acute brucellosis were preliminarily compared, and the reasons of the differences were analyzed. Results Among the 20 serum samples from patients with acute brucellosis, 20 were positive by digital PCR and 0 by fluorescent quantitative PCR. Conclusions It is preliminarily confirmed that the sensitivity of digital PCR is higher than that of fluorescent quantitative PCR in the detection of serum samples from patients with acute brucellosis. It has good clinical application prospects, but it also has some limitations. It is necessary to further expand the sample to complete the clinical research of experimental diagnosis and carry out multi-laboratory validation.     

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备注/Memo

备注/Memo:
[ 基金项目] 北京市属医院科研培育计划(PX2016019,PX2019065); 首都医科大学附属北京地坛医院院内科研基金项目(DTQL201801)
[ 通信作者]  蒋荣猛,E-mail: 13911900791@163.com
更新日期/Last Update: 2019-09-15