|本期目录/Table of Contents|

[1]修 敏,任妍妍.实时荧光定量PCR 与 细胞培养法在流感病毒检测中的比较[J].传染病信息,2019,04:336-337+340.
 XIU Min,REN Yan-yan.Comparison of real-time fluorescence quantitative PCR with cell culture in detection of influenza virus[J].Infectious Disease Information,2019,04:336-337+340.
点击复制

实时荧光定量PCR 与 细胞培养法在流感病毒检测中的比较(PDF)

《传染病信息》[ISSN:1007-8134/CN:11-3886/R]

期数:
2019年04期
页码:
336-337+340
栏目:
论著
出版日期:
2019-09-12

文章信息/Info

Title:
Comparison of real-time fluorescence quantitative PCR with cell culture in detection of influenza virus
文章编号:
1007-8134(2019)04-0336-03
作者:
修 敏任妍妍
123000,阜新市卫生健康服务中心(阜新市疾病预防控制中心)检验检测部(修敏、任妍妍)
Author(s):
XIU Min REN Yan-yan
Inspection and Detection Department of Fuxin Health Service Center (Fuxin Center for Disease Control and Prevention), 123000, China   
关键词:
流感病毒检测细胞培养实时荧光定量PCR
Keywords:
influenza virus detection cell culture real-time fluorescence quantitative PCR
分类号:
R248.4 
DOI:
10.3969/j.issn.1007-8134.2019.04.012
文献标识码:
A
摘要:
目的 探讨实时荧光定量PCR 与细胞培养法在流感病毒检测中的应用效果。方法 将我中心流感高峰季节接 收的流感样病例样本726 例分别采用实时荧光定量PCR 和细胞培养检测方法进行检测,分析2 种检测方法的阳性检出率和 实时荧光定量PCR 方法的灵敏度和特异度。结果 726 例流感样病例样本中,细胞培养阳性89 例,阳性率12.26%;实时荧 光定量PCR 阳性189 例,阳性率26.03%。以细胞培养法为金标准,计算实时荧光定量PCR 检测法灵敏度为91.01%,特异 度为83.05%。结论 实时荧光定量PCR 具有灵敏度高、检出速度快等特点,可广泛应用于临床诊断、常规监测以及流感疫 情应急检测等。
Abstract:
Objective To investigate the application of real-time fluorescence quantitative PCR and cell culture in influenza virus detection. Methods Seven hundred and twenty-six samples of influenza-like cases received in the peak season of influenza in our center were detected by real-time fluorescence quantitative PCR and cell culture detection methods respectively. The positive detection rate of the 2 methods and the sensitivity and specificity of real-time fluorescence quantitative PCR were analyzed. Results Of 726 influenza-like cases, 89 were positive in cell culture (12.26%) and 189 were positive in real-time fluorescence quantitative PCR (26.03%). Taking cell culture method as golden standard, the sensitivity and specificity of real-time fluorescence quantitative PCR were 91.01% and 83.05% respectively. Conclusions Real-time fluorescent quantitative PCR has a high sensitivity and rapid detection. It can be widely used in clinical diagnosis, routine monitoring and emergency detection of influenza.  

参考文献/References


[1] 薛京昌,卞琛,徐晓怡. 实时荧光定量PCR 法在流感病毒 暴发疫情病原学检测诊断中的应用[J]. 实用预防医学, 2017,24(5):625-626.
[2] 赵孟孟,冯松林,王文佳,等. Nsp9 基因实时荧光定量PCR 检测方法的建立及其在感染细胞过程中表达量的变化[J]. 中国畜牧兽医,2016,43(10):2534-2540.
[3] 刘婷婷,谢芝勋,宋德贵,等. H3 和H4 亚型禽流感病毒 双重实时荧光定量PCR 检测方法的建立[J]. 中国家禽, 2015,37(7):29-34.
[4] 魏明琳. 流感病毒分型与亚型实时荧光定量PCR 检测能力验证 结果分析[J]. 临床检验杂志(电子版),2017,6(2):393-394.
[5] 国家卫生健康委员会. 流行性感冒诊疗方案 (2018 年版修订 版)[J]. 传染病信息,2018,31(6):500-504.
[6] 魏明琳. 流感病毒分型与亚型实时荧光定量PCR 检测能 力验证结果分析[J]. 临床检验杂志(电子版),2017, 6(2):393-394.
[7] 付运星,吴永丽,徐道修,等. 犬瘟热病毒一步法荧光定量 PCR 检测方法的建立[J]. 中国兽医学报,2016,36(3):407- 411.
[8] 杨燕,单万水,韩红星,等. 化学发光法和实时荧光定量 PCR 法检验EB 病毒的临床效果对比[J]. 国际免疫学杂志, 2016,39(5):463-465.
[9] 潘超,韦天超,农海连,等. 鸽白细胞介素8 基因实时荧 光定量PCR 检测方法的建立[J]. 中国畜牧兽医,2016, 43(10):2547-2552.
[10] 孙文超,解长占,赵冠宇,等. 犬圆环病毒实时荧光定量 PCR 检测方法的建立[J]. 动物医学进展,2017,38(5):6-10.
[11] 邸红芹, 杨永辉, 朱桂云, 等. 改良多重实时荧光定量 PCR 检测流感A 型和B 型病毒[J]. 河北医药,2016, 38(23):3529-3532.
[12] 李丹丹,徐义刚,李梦圆,等. 单核细胞增生李斯特氏菌实 时荧光定量PCR 快速检测方法的建立[J]. 中国畜牧兽医, 2016,43(6):1453-1457.
[13] 赵鸿,李妍,戎国栋,等. 实时荧光定量PCR 检测外周血 白细胞和血浆EB 病毒DNA 载量[J]. 江苏医药,2017, 43(7):509-511.

备注/Memo

备注/Memo:
-
更新日期/Last Update: 2019-09-15